A monopartite sequence is essential for p45 NF-E2 nuclear translocation, transcriptional activity and platelet production
نویسندگان
چکیده
منابع مشابه
Human MafG is a functional partner for p45 NF-E2 in activating globin gene expression.
Mammalian globin gene expression is activated through NF-E2 elements recognized by basic-leucine zipper proteins of the AP-1 superfamily. The specificity of NF-E2 DNA binding is determined by several nucleotides adjacent to a core AP-1 motif, comprising a recognition site for transcription factors of the Maf subfamily. Earlier work proposed that p18(MafK) forms a heterodimer with hematopoietic-...
متن کاملGenetic analysis of hierarchical regulation for Gata1 and NF-E2 p45 gene expression in megakaryopoiesis.
GATA1 and NF-E2 p45 are two important regulators of megakaryopoiesis. Whereas GATA1 is known to regulate the p45 gene, details of the GATA1 contribution to the spatiotemporal expression of the p45 gene remain to be elucidated. To clarify the relationship between GATA1 and p45, we performed genetic complementation rescue analysis of p45 function in megakaryocytes utilizing the hematopoietic regu...
متن کاملRAPID COMMUNICATION Human MafG Is a Functional Partner for p45 NF-E2 in Activating Globin Gene Expression
Mammalian globin gene expression is activated through NFthat encodes the human homolog of chicken MafG. Human MafG heterodimerizes with p45 NF-E2 and binds DNA with E2 elements recognized by basic-leucine zipper proteins of the AP-1 superfamily. The specificity of NF-E2 DNA binding specificity identical to that of purified NF-E2 DNA binding activity. A tethered heterodimer of p45 and MafG is fu...
متن کاملMultiple regions of p45 NF-E2 are required for beta-globin gene expression in erythroid cells.
Regulated expression of genes in the beta-globin cluster depends upon sequences located between 5 and 20 kb upstream of the epsilon gene, known as the locus control region (LCR). beta-Globin expression in murine erythroleukemia (MEL) cells depends on NF-E2, a transcription factor which binds to enhancer sequences in the LCR. To gain insight into the mechanism of globin gene activation by NF-E2,...
متن کاملThe transcriptional integrator CREB-binding protein mediates positive cross talk between nuclear hormone receptors and the hematopoietic bZip protein p45/NF-E2.
Thyroid hormone (T3) and retinoic acid (RA) play important roles in erythropoiesis. We found that the hematopoietic cell-specific bZip protein p45/NF-E2 interacts with T3 receptor (TR) and RA receptor (RAR) but not retinoid X receptor. The interaction is between the DNA-binding domain of the nuclear receptor and the leucine zipper region of p45/NF-E2 but is markedly enhanced by cognate ligand. ...
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ژورنال
عنوان ژورنال: Journal of Thrombosis and Haemostasis
سال: 2010
ISSN: 1538-7933
DOI: 10.1111/j.1538-7836.2010.04058.x